Liver cancer is the second leading cause of cancer-related death worldwide, so early detection and prediction for response to treatment is of great benefit to hepatocellular carcinoma (HCC) patients. Currently, needle biopsy and conventional medical imaging play a significant and basic role in HCC patients' management, while those two approaches are limited in sample error and observer-dependence. Radiomics can make up for this deficiency because it is an emerging non-invasive technic that is capable of getting comprehensive information relevant to tumor situation across spatial-temporal limitation. The basic procedure for radiomics includes image acquisition, region of interest segmentation and reconstruction, feature extraction, selection and classification, and model building and performance evaluation. The current advances and potential prospect of radiomics in HCC studies are involved in diagnosis, prediction for response to treatment, prognosis evaluation and radiogenomics.
The article entitled "Differential expression of exosomal miRNAs in osteoblasts in osteoarthritis" published on Journal of Central South University (Medical Science), in Volume 43, Issue 12, 2018 (DOI: 10.11817/j.issn.1672-7347.2018.12.003) may have an unclear risk of bias due to insufficient understanding for some results. Further experimental studies are needed. We all agree to retract this article, and apologize to the Journal and readers for the possible negative impact.
To explore the role of prostaglandin E2 (PGE2) and epidermal growth factor (EGF) in the protective effect of polaprezinc on acute gastric mucosal damage. Methods: A total of 30 male SD rats were randomly divided into 3 groups as follows: A blank group, a model group, and a polaprezinc group. The blank group was fed normally. As for the model group and the polaprezinc group, the rats were given 1 mL ethanol (intragastrical administration) first, then they were treated with vehicle (1 mL distilled water) or polaprezinc treatment (100 mg/kg) 1 h later. On the 3rd day, rats in each group were fasting for 24 h before gastric administration. After 2 h of gastric administration, 5 mL of intraperitoneal blood was collected, centrifuged and stored at -80 ℃ for the detection of EGF and PGE2. Gastric tissue was collected for anatomic and pathological assessment. Results: Polaprezinc reduced gastric mucosa injury in the polaprezinc group compared to the model group. Compared with the blank group, the levels of PGE2 and EGF in blood serum were significantly decreased in the model group and the polaprezinc group (P0.05). Conclusion: Polaprezinc can provide effective protection for acute mucosal injury and the underlying mechanism is not directly related to PGE2 and EGF.
Systemic sclerosis (SSc) is an autoimmune disease characterized by thickening of the skin and organ fibrosis. Ankylosing spondylitis (AS) is a type of arthritis with long-term inflammation of the axial joints. Previous studies presented 5 cases of concomitant AS and SSc. However, there was only 1 patient of those 5 cases complaining of muscle weakness while all patients had approximately normal creatine kinase (CK). Here we reported a young male who met the criteria for SSc and AS while showing significantly elevated CK. Human leukocyte antigen (HLA) typing results indicated the genetic susceptibility to these two diseases. The patient was prescribed prednisone (30 mg/d) and cyclophosphamide. After 2 months, the patient's skin became soft with normal CK.
Dermatomyofibroma is a benign and rare proliferation of myofibroblasts and fibroblasts of the skin. Dermatomyofibroma commonly locates at the shoulder and neck of young adults and adolescents. Other frequently affected anatomic sites are upper arms, thigh, chest wall, back, axillary region and abdomen. Herein, we reported a case of dermatomyofibroma occurred in the nasion. The asymptomatic firm nodule and histopathological features were consistent with dermatomyofibroma. Immunohistochemically, the tumor cells expressed vimentin, HHF35 and α-smooth muscle actin (α-SMA). The patient was followed up for 2 years after excision of the tumors and recurrences were not observed.
To evaluate the feasibility and safety of device closure of patent ductus arteriosus (PDA) using only venous access under echocardiography guidance alone. Methods: A total of 102 consecutive pediatric patients underwent transcatheter PDA closure without arterial access, under the guidance of only echocardiography. The patients were followed up by clinical examination, electrocardiogram, and echocardiogram at 1, 3, 6 12, and 24 months. Results: Transvenous PDA closure under echocardiographic guidance was successful in 99 (97.1%) patients. There were no acute procedural complications or severe adverse events. The duration ranged from 10 to 65 minutes (median, 21 minutes). Immediate complete closure of PDA was achieved in 87 patients (87.9%), and 100% of the patients were completely closed after 24 h. There were no severe adverse events in the period of 1-24 months (median, 12 months) follow up. Conclusion: Transvenous PDA closure without fluoroscopy avoids radiation exposure, contrast agent usage and potential arterial complications. It can be used as an alternative procedure, especially for children.
Gliomas are the most common brain primary tumors worldwide, which is the earliest sequenced cancer gene in the Cancer Genome Atlas (TCGA) project. The World Health Organization Classification Update of Central Nervous System (CNS) Tumors 2016 highlights that glioma is the first tumor classified based on both of the molecular markers and histology. Radiomics is an extraction approach for high-throughput data which collects the quantitative image information appearing. Combined imaging data with genomics and proteomics, radiomics show promising prediction for cancer diagnosis, treatment, and prognosis. In this review, the radiomic analysis methods applied in gliomas are highlighted. Some remarkable findings confirm the considerable potential of radiomics in clinical cancer research.
To compare the anterior temporal lobectomy (ATL) with transsylvian selective amygdalohippocampectomy (SeAH) in 72 patients with medial temporal lobe epilepsy (MTLE) regarding the seizure control and neuropsychological outcomes. Methods: Clinical data and follow-up data were collected and retrospectively analyzed. SeAH and ATL were used in 39 and 33 patients, respectively. All eligible patients were followed up at least one year. Wechsler Adult Intelligence Scale-Revised and the Wechsler Memory Scale-Revised were used to test the patients' neuropsychology before and after the surgery for one year. Results: Fifty-nine patients (81.9%) achieved satisfactory seizure control (62.5% Engel Class I and 19.4% Class II). ATL obtained 84.8% satisfactory seizure control (28 patients), and the success rate was 79.5% (31 patients) for SeAH. There was no significant difference in seizure control between SeAH and ATL (P=0.760). The postoperative verbal IQ of SeAH group increased significantly in both side surgery (P0.05). Regarding left-side surgery, postoperative verbal memory and total memory were increased significantly in the group of SeAH (P0.05). In the right-side surgery, postoperative verbal memory and total memory were increased significantly in the two surgery strategy groups (P0.05). Conclusion: Microsurgery for the treatment of refractory MTLE is successful and safe, and should be encouraged. The seizure outcome is not different between ATL and SeAH, while regarding as verbal IQ and verbal memory outcomes, SeAH may be superior to ATL in dominant hemisphere surgery.
To investigate the potential correlation between miR-223 level in leukocytes and platelet responses to clopidogrel in patients with coronary artery disease. Methods: A cohort of 188 outpatients, who conducted percutaneous coronary intervention (PCI) and received dual antiplatelet therapy, were recruited. The patient's electronic health data were collected, and their blood samples were obtained for measurement of adenosine diphosphate (ADP)-induced whole-blood platelet aggregation. Extreme cases of platelet responses to clopidogrel (ultra- vs. non-responder) were measured with miR-223-3p levels in leukocytes. Results: Both groups had similar miR-223-3p levels in leukocytes. There were no significant differences in other demographic and clinical data except for metrics of ADP-induced whole-blood platelet aggregation between the 2 group. Conclusion: MiR-223-3p in peripheral leukocytes is not associated with the altered platelet responses to clopidogrel in PCI outpatients.
This report summarized current knowledge and findings relevant to environmental and genetic risk factors in brain tumors, with a particular focus on glioma. To date, the established risk factors for brain tumors are family history and ionizing radiation exposure; whereas there is an inverse association between tumors and other factors such as history of allergies, atopic conditions, chickenpox, and varicella zoster virus infection. To identify inherited genetic variants impacting susceptibility of brain tumors, large scale familial linkage-scan pedigree analysis, population-based candidate genes, and genome-wide association study were performed. More recently, next generation exome and whole genome sequencing studies were also conducted.
To seek survival-related genes in glioblastoma and establish a survival-gene signature for predicting prognoses of glioblastoma using public databases. Methods: Three independent glioma databases (GEO GSE53733, CGGA, TCGA) with whole genome expression data were included for analysis. Survival-related genes were obtained by comparing the long-term (>36 months) and short-term (<12 months) survivors in the database GSE53733. CGGA was used as the training set to develop the signature and TCGA was used as the validation set. Cox regression analysis and linear risk score assessment were conducted to look for prognostic signatures with survival-related genes. Principal components analysis, gene set enrichment analysis (GSEA), gene ontology (GO) and protein-protein interaction (PPI) analysis were performed to explore distinct expression profiles between risk grouped glioblastoma. Results: We totally found 211 survival-related genes and developed a signature with 17 survival-related genes for prognosis of glioblastoma. Based on this signature, the low-risk group had longer survival time while the high-risk group had shorter survival time. Additionally, the expression profiles between the high-risk and low-risk glioblastoma were different. Functional annotations revealed that the genes enriched in the high-risk glioblastoma were involved in immune systems and processes of extracellular matrix (ECM). Conclusion: The novel survival-gene signature can predict high-risk glioblastoma with shorter survival time, enhance immunosuppressive features, and increased invasion preferences.
To determine associations between postpartum depression (PPD) and plasma neurotransmitters. Methods: We conducted a case-control study nested to a prospective cohort established in 3 comprehensive tertiary hospitals in Changsha, Hunan, China from February to September 2007. The Chinese version of the Edinburgh Postnatal Depression Scale (EPDS) was used at 2 weeks postpartum to screen PPD, with a score of 13 or higher as the cut-off for PPD. The women with matched age but without PPD and delivery within 5 years were selected as controls. The levels of plasma monoamine neurotransmitters including serotonin (5-hydroxytryptamine, 5-HT), dopamine (DA), and norepinephrine (NE), and peptide neurotransmitters including neuropeptide Y (NPY) and substance P (SP) in maternal blood samples taken at 2 weeks postpartum were measured and compared between PPD women (n=42) and controls (n=42). Results: Plasma levels of 5-HT and NPY were significantly lower while plasma levels of NE and SP were significantly higher in PPD women than those in the controls. For women with PPD, a negative correlation between NPY and NE (r=-0.36, P﹤0.05) was observed. Conclusion: There are changes in plasma levels of neurotransmitters in women with PPD, and there are potential interactions between different neurotransmitters.
To investigate associations of interleukin-31 (IL-31) and pruritus in atopic dermatitis (AD) with Meta-analysis. Methods: Materials were extracted from the citations listed in the following databases: PubMed, Science Direct, Web of Science and Cochrane. Key search terms included: atopic dermatitis, pruritus, and IL-31. The Meta-analysis was used to analyze the correlation between pruritws in AD and IL-31 expression level. Results: The Meta-analysis showed that serum IL-31 levels were higher in AD patients than those in the healthy controls. The levels of IL-31 were higher in severe AD patients than those in the mild and moderate AD patients. Moreover, a positive correlation between serum IL-31 levels and severity of pruritus was identified. Conclusion: Increased serum levels of IL-31 generally exist in the AD patients, and it may accelerate the pruritus in the AD patients.
Ozone was discovered in the mid-nineteenth century and is proven to have many therapeutic effects, including its common application as a disinfectant to kill microorganisms in various conditions. Ozone therapies have been utilized for various purposes ever since it was discovered. Extensive studies over a century have verified its therapeutic effects, consistency, and safety with minimal and preventable side effects in medical care. Emerging evidence revealed that ozone also plays important roles in the management and prevention of various skin disorders including infectious skin diseases, skin related allergic diseases, erythema scaly diseases, wound healing and ulcer recovery. Herein, the author now summarizes the recent clinical applications of ozone therapy in dermatology and provide commentary on what we have learned in our practice. Our focuses are the efficacy and safety of ozone therapies as well as the application prospects of ozone on various skin disorders. In addition, the author discusses the potential mechanisms involved in ozone therapy and the efforts we should make for.
To evaluate efficacy of combined therapy with ozonated water and oil on patients with tinea pedis. Methods: A total of 60 patients with tinea pedis were divided into 2 groups in a randomized and blinded test. Patients in a control group were treated with naftinfine hydrochloride and ketoconazole cream once a day. Patients in an ozone group were treated with ozonated water bath and then ozonated oil topical application once a day. Patients in the 2 groups were treated for 4 weeks. Clinical and laboratory data were collected for both groups at the end of the 1st week, the 2nd week, and the 4th week. The Pearson chi-square was performed to compare scores of the clinical signs and symptoms (CSS) and the mycological result between the 2 groups. Independent samples T-test was performed to compare the curative effect between the 2 groups. Results: After 4 weeks' treatment, 6 patients were positive in the control group determined by mycological examination while 1 patient was positive in the ozone group, with no significant difference between the 2 groups (P>0.05). Changes in CSS at the end of the 1st week, 2nd week, and 4th week were obtained and showed no significant difference between the 2 groups at the 3 different time points (P>0.05). No side effects were observed. Conclusion: Combination of ozonated water with oil is effective on treatment of tinea pedis and it shows no side effects.
To enhance the understanding of the left coronary artery originating from the pulmonary artery, we report a case of sudden cardiac arrest during exercise. After successful cardiopulmonary resuscitation, the patient underwent echocardiography and selective coronary angiography, and the disease was firmly diagnosed. Consequently, a thoracic surgery was performed. During the operation, the left coronary artery was transplanted to the root of the aorta and the pulmonary valve and artery were reconstructed. Finally, the surgery was successful.
To understand Chinese gay men's health and life satisfaction in Guanzhou, and to analyze the main factors for life satisfaction. Methods: Male gay participants were recruited through respondent-driven sampling and face-to-face investigations with self-administered questionnaires, including a general demographic questionnaire, a suicide questionnaire, the Depression-Anxiety Stress Scale, the Self-Stigma Scale, the Perceived Social Support Scale, the Rosenberg Self-Esteem Scale, and the Satisfaction with Life Scale. Results: Surveys were successfully conducted for 420 gay men. High levels of depression, anxiety, and stress were observed among the respondents. A total of 29.3% had suicidal tendency in the past year, 36.5% experienced dating violence, 39.2% had homosexual sex without protection in the past 6 months, and 14.0% were infected with at least one type of infection of sexually transmitted diseases. Multiple linear regression analysis showed that gay men who felt highly satisfied with life exhibited low levels of self-stigma, high self-esteem, and low levels of depression. Moreover, they did not suffer dating violence and playing passive sex roles. Conclusion: The survey confirmed that gay men in Guangzhou, China, have poor health and lower life satisfaction levels. Self-stigma, self-esteem, sex roles, depression, and dating violence are the main factors that influence the life satisfaction of them.
To illuminate a method for establishment of a cost-efficient atopic dermatitis (AD) mouse model by topical application of ovalbumin (OVA), super-antigen staphylococcal enterotoxin B (SEB), and calcipotriene ointment (CO) on the back of BALB/c mice. Methods: Experimental mice were topically treated with OVA/SEB or OVA/SEB/CO every other day during 15 days of induction. Clinical alterations on the skin area were monitored every other day. Epidermal thickness were measured by reflectance confocal microscope (RCM) before harvest. Inflammatory cells in skin biopsies were marked by hematoxylin-eosin (HE) staining. Blood sample and skin biopsies were measured by ELISA and quantitative real-time PCR to detect the expression of IL-2, IL-4, IL-31, interferon (IFN)-γ, tumor necrosis factor (TNF)-α pruritus-associated nerve growth factor (NGF), and serum IgE. Results: Human AD-like cutaneous local inflammatory reaction was characterized by the accumulation of inflammatory cells, increased epidermal thickness and serum IgE levels as well as Th1 cell-associated cytokines (IFN-γ, TNF-α), Th2 cell-associated cytokines (IL-4, IL-31), and NGF in the OVA/SEB/CO group compared with that in the normal control group or the OVA/SEB group. Conclusion: OVA/SEB/CO can induce an AD-like mouse model with lower economic and time consumption.
To analyze the trend relevant factors leading to death and their patterns over a 10-year period in inpatients with connective tissue diseases (CTDs). Methods: All clinical data about death in inpatients with CTDs were retrospectively reviewed between 2005 and 2014 at the Department of Rheumatology and Immunology in Xiangya Hospital of Central South University. Results: In the 10-year time period, the overall hospital mortality was 15.68‰. The disease itself accounted for 44.71% of the total causes of death, infection accounted for 42.94%, and comorbidities accounted for 12.35%. The constituent ratio of deaths and the average hospital mortality caused by the disease itself declined gradually year by year, and the constituent ratio of deaths caused by infection and comorbidities increased gradually year by year (P<0.05). In 2013-2014, infection was the leading cause of death, which accounted for 51.06%. The survival time for CTDs inpatients with interstitial lung disease (ILD) was shorter than that of CTDs inpatients without ILD, and even the risk of death was 1.722 times of the latter. The proportion of deaths caused by the disease itself was the highest in systemic sclerosis and systemic lupus erythematosus, that by infection was the highest in idiopathic inflammatory myopathy (IIM), and that by comorbidities was the highest in rheumatoid arthritis. Conclusion: The proportion of deaths and the hospital mortality in CTDs inpatients caused by the disease itself show a declining trend, while the proportion of deaths caused by infection and comorbidities increase. CTDs patients with ILD have shorter survival time and an increase in risk of death.
Translation control in eukaryotes contributes significantly to gene expression regulation during cellular processes, which enables rapid changes of specific proteins to maintain cellular homeostasis. Eukaryotic translation is a multiple-step process that comprised of four phases: initiation, elongation, termination and ribosome recycling. The initiation phase is rate-limiting and orchestrated by a set of eukaryotic translation initiation factors (eIFs). Defects in translation initiation can result in a series of diseases. Among all eIFs, eIF3 is the largest and less-known initiation factor due to its intrinsic complexity. Aberration in eIF3A, the largest subunit of eIF3, is known to contribute to carcinogenesis and protection against evolution into higher-grade malignancy, and the altered expression or mutation of eIF3A affects the responses of cancer patients to platinum-based chemotherapy. Besides its role in cancinogenesis, eIF3A is also implicated in fibrosis, and the agents inhibiting eIF3A delay the progression of this disorder. The dual roles of eIF3A in tumorigenesis are probably due to the regulation of translation of different mRNAs at different stages of tumor progression by eIF3A. In turn the encoded products serve as pro-tumor or anti-tumor proteins at different stages.