To observe the abnormal refractive state and clinical characteristics in preterm and full-term children in the department of pediatric ophthalmology. The ocular refraction status of 358 eyes in 51 preterm and 128 full-term children were checked by retinoscopy in dilated pupil after being used atropine eye drop． There were 157 eyes with hyperopia accounting for 61.3% in preterm children, 25 eyes with hyperopia accounting for 24.5% in full-term children. The main type of refractive errors in preterm children was astigmatism, especially high-astigmatism, mixed astigmatism. And the morbidity of astigmatism, hyper-astigmatism, mixed astigmatism in preterm children was higher than those in full-term children. Astigmatism, especially high astigmatism and complex mixed astigmatism are important reasons for low vision in preterm children. Clinically, there is close relationship between astigmatism and amblyopia. So it can not be ignored with preterm children's visual development, and refractive screening could be brin
To evaluate the reading speed of adolescents with different causes of low vision using electronic visual aids. The screening of 10 young students from Quanzhou Blind School could read Chinese N5 print by different optical aids. After their refractive correction and ophthalmic examination, the reading speeds with Chinese N5 print were measured using various optical and electronic visual aids. The reading speed of (34.36±5.06) word / min by electronic visual aids performed faster than that of (18.50±6.54) word / min by optical visual aids in 10 young students with low vision (P0.05）. The electronic visual aids more significantly improve the reading speed of young people with low vision than the optical visual aids did. The reading speed of young people with low vision is not relevant to their near visual acuity induced by different causes.
To study the reliability of digital slit lamp microscope as a tool for quantitative analysis in one dimensional size measurement. Three single-blinded observers acquired and repeatedly measured the images with a size of 4.00 mm and 10.00 mm on the vernier caliper, which simulatated the human eye pupil and cornea diameter under China-made digital slit lamp microscope in the objective magnification of 4 times, 10 times, 16 times, 25 times, 40 times and 4 times, 10 times, 16 times, respectively. The correctness and precision of measurement were compared. The images with 4 mm size were measured by three investigators and the average values were located between 3.98 to 4.06. For the images with 10.00 mm size, the average values fell within 10.00 ~ 10.04. Measurement results of 4.00 mm images showed, except A4, B25, C16 and C25, significant difference was noted between the measured value and the true value. Regarding measurement results of 10.00 mm iamges indicated, except A10, statistical significance was found be
To report target z-axis optimized LASIK treating for two eyes from two patients with decentered ablation. The LaserSight AstraPro2.2 Z software is a topography-guided custom ellipsoid ablation platform. LASIK with target z-axis optimization was performed to restore pre-operative visual axis using a LaserSight SLX excimer laser (version 5.3,300Hz). During preoperative examination, the uncorrected visual acuity (UCVA) was 0.5 in both eyes, and the best spectacle-corrected visual acuities(BSCVA) were 1.0 and 0.8. The decentered ablation zones were diagnosed with LaserSight AstraMax topography. Following target z-axis microtranslation, fifteen and eight μm of central corneal tissues were preserved in the two eyes, respectively. The UCVA was 1.2 in both eyes, and secondary ablation zones were both centered on the visual axis. Topography maps and UCVA were stable in both eyes at the time of final follow-up. Using target z-axis optimized LASIK with the LaserSight AstraPro Planner 2.2 Z customized ablation software w
To establish a simple and convenient method for the culture of human umbilical vein endothelial cells (HUVECs) and study its characterization in vitro. Human Umbilical cord was isolated and digested by collagenase type I,and then it was cultured in 1.5% geltain coated dish with 10% fetal bovine serum(FBS)，heparin sodium andβ-endothelial cell growth factor(β-ECGF) in human endothelial basal growth medium．HUVECs were identified by anti-human factor Ⅷ related antigen and CD31 immunohistochemical staining． We could successfully culture HUVECs by using this method. HUVECs attached the dish in 24 hours and the confluence was seen in 5 days. HUVECs were generally positive for anti-human factor Ⅷ related antigen and CD31． It is a fast and effective method to successfully culture purified HUVECs in which collagenase type I was used to digest HUVECs with glass tube connected to T-tube, human endothelial basal growth medium containing 10% FBS,heparin sodium and β-ECGF in 1.5% geltain coated dish.
To analyze the retinal symmetry of multifocal visual evoked potential (mfVEP) in both eyes of normal subjects. The monocular mfVEP in both eyes of 36 normal subjects (72 eyes) were tested with VERIS Science 4.0. The stimulus was the pattern reversal dart array consisted of 60 sectors, each included 16 black-white reverse patterns. The visual stimulation was controlled by a binary pseudo-random m-sequence and subtended 25 degrees of retinal region. The mfVEP patterns between left and right eyes of each subject were similar, and P1 latency and amplitude in correspondent visual field quadrants between left and right eyes had no significant difference(P>0.05). The latency of superotemporal visual field quadrant in right eyes was shorter than that of superonasal visual field quadrant in left eyes, and the amplitude of superonasal visual field quadrant in right eyes was longer than that of superotemporal visual field quadrant in left eyes(P<0.05). The P1 latency and amplitude among four visual field quadrants of ea
To investigate the clinical cause, therapy and prognosis of shallow anterior chamber after complex trabeculectomy surgery of glaucoma. A retrospective study of 267 patients (302 eyes) diagnosed with glaucoma at our hospital were reviewed. Forty-three among 302 eyes presented shallow anterior chamber（14.24%）.The main causes of shallow anterior chamber after surgery included excessive filtering function, conjunctival exudation, choroidal detachment and ciliary circle block. These patients recovered after receiving conservative or surgical treatment. The shallow anterior chamber is a complication frequently occurring following complex trabeculectomy surgery. The occurrence of this complication may be decreased by controlling filterableness and moderately applying mitomycin C intraoperatively; The prognosis may be significantly enhanced by detecting main measurements including anterior chamber, intraocular tension, filtration condition,and by interfering in shallow anterior chamber early after complex trabeculect
To study the curative effect of amnion membrane transplantation on decreasing corneal neovascularization(CNV) induced by alkali burn. It was a non-randomized retrospective case-control study. Among 19 cases (23 eyes) of third-degree alkali burns from 2006 to 2010, 11 cases (13 eyes) were performed with amnion membrane transplantation operation, and others were not. Amnion membrane transplantation was performed at 3rd day after burns in the treatment group. Ages and treatments beyond surgery of double groups were matched. Areas of CNV in double groups were measured at the 14th and 60th days after burn. RESULTS：Area of CNV in the treatment group was (62.133±8.571) mm² at the 14th day after burn, and was 30.6% lower than that in the control group. Area of CNV in the treatment group was (112.019±17.362)mm² at the 14th day after burn, and was 13.5% lower than that in the control group. There was statistical significance between the two groups (P<0.05). Amnion membrane transplantation operation can inhibit the grow
To study the characteristics of photopic flash electroretinogram (ERG) in normal adult SD rats. Sixty normal adult SD rats aged 9 to 12 weeks were enrolled in this study. Photopic flash ERG were recorded from these 60 SD rats. The results were statistically analyzed with SPSS software. Stable a, b and PhNR wave could be recorded in each rat. Implicit time of a wave, implicit time and amplitude of PhNR fit normal distribution. Implicit time of PhNR was (124.6±8.5) ms with the smallest coefficient of variation of 0.07. The amplitude of PhNR was (11.3±4.2) μV and coefficient of variation was 0.37. There was no difference in the results between female and male rats. Photopic flash ERG is an objective method in evaluating the retinal function in SD rats and PhNR can be used as a sensitive index of inner retinal function.
Steroid-induced glaucoma in rabbit ocular was established to observe the ultrastructural changes in trabecular meshwork,and to preliminarily study the mechanisms of increased resistance of aqueous outflow in steroid-induced glaucoma. Forty New Zealand white rabbits were randomly divided into four groups, namely,control group (n = 10 rats),drops drug group(n = 10 rats), Injecting drug group(n = 10 rats) and drug combination group (n=10 rats). For three consecutive days before the experiment,using the Schitoz's tonometer to measure intraocular pressure(IOP) at 8am, 10am, and 12am each day,obtained the average,and finally calculated basis IOP of these rabbits. The rabbits eyes in drug combination group of were treated with dexamethasone sodium phosphate solution (0.5%)for 8 weeks,three times a day, and given injection of triamcinolone endure (3 mg) weekly. While the rabbits eyes in the control group were treated with sterile saline in the same period of experiment.After 4 days,IOP of the rabbit was measured by S
To investigate the inhibitory effect of diclofenac sodium on rabbit corneal epithelial cells (RCECs) in vitro and explore its pharmacological mechanism. The fresh rabbit cornea was cultured to obtain the primary RCECs, and RCECs of passage 2 were used in this research. The cells were divided into experimental groups, the cells in which were incubated with different concentrations (18.18, 27.27, 36.36, 45.45, 54.55 μg/ml) of diclofenac sodium, and control group. The effect of diclofenac sodium on the proliferation of cells was measured by methyl thiazolyl thiazolium (MTT) assay 24, 48 and 72 h after incubation. While the RCECs were divided into experimental groups, the cells in which were incubated with 9 and 12.5 μg/ml diclofenac sodium, and control group. The cell cycle and apoptotic rate were observed by flow cytometer. MTT assay showed that diclofenac sodium had obvious inhibitory effect on RCECs, and the inhibition rate was increasing along with the increasing concentration of diclofenac sodium and the in
To investigate the clinical effect of anastomosis lacrimal sac conjunctival sac in the treatment of severe laceration of lacrimal canaliculus. A total of 19 cases (19 eyes) with laceration of lacrimal canaliculus underwent anastomosis lacrimal sac conjunctival sac.All the patients were followed up for 3 to 24 months postoperatively. Among all the 19 patients (19 eyes), 14 cases were cured,3 cases wre markedly improved and 2 cases had no effect,and the effect rate was 89.47%. Anastomosis lacrimal sac conjunctival sac is an effective surgical technique in the management of severe laceration of lacrimal canaliculus.
To compare the advantages and disadvantages of GMS and PAS Staining in the diagnosis of fungal keratitis. Retrospectively analysed the sections of 102 cases of fungal keratitis from November 2007 to June 2010 in our department. Statistically analysed the positive rate of different statining methods, and summarized technical cruxes of GMS and PAS staining. The positive rates of GMS and PAS staining for fungal keratitis were 100% and 93.14%, respectively. GMS staining is better than PAS staining for fungal keratitis in displaying fungi. And it takes less time, is easier to operate and presents a more durable colour compared with PAS staining. But PAS staining is superior to GMS staining in displaying fungal septa and eliminating interference from pigment-containing cells and other cells.
To investigate the effect of intravitreal basic fibroblast growth factor(bFGF) on activation and proliferation of endogenous retinal progenitor cells in the Royal College of Surgeons(RCS) rats. Twenty-four rats were studied after the 30th postnatal day(≥30). Eighteen affected rats were randomly divided into 3 groups: bFGF-treated, vehicle-treated and untreated group, and 6 unaffected rats were used as normal controls. Six μl of bFGF (5μg/10 μl) or vehicle was injected into the vitreous on days 31, 33 and 35 after birth (P31, P33, P35) in the bFGF group and vehicle group, and no injection was administered in the untreated and control groups. All the rats were euthanized, and their eyes were enucleated, hemisected and fixed at 50 d after birth for immunohistochemistry and measurement of outer nuclear layer thickness. Nestin and Chx10 were positively expressed in all retinal layers, intravitreous injection of bFGF in retina-dystrophic RCS(RCS-p+/Lav) rats induced intense labeling for the retinal progenitor cell
To observe the changes of ocular fundus and to describe the image features of optical coherence tomography (OCT) in patients with laser-induced maculopathy. Four patients (4 eyes) with decreased visual acuity due to laser injury were recruited into the study between April 2002 to December 2009. All patients underwent routine check-up for eyes. The four cases presented with different clinical characteristics from each another. In the first case, OCT showed lamella macular hole, with partially remained outer sensory retina. The signals of inner segments/outer segments (IS/OS) band and RPE band were disrupted. The choroidal band was distorted with increased reflection. Perimacular epiretinal membrane and vitreous hemorrhage were also observed. The second case had a full-thickness macular hole with retina edema surrounding the hole. The third case presented local serous sensory retinal detachment in macula. The fourth case presented hyper-reflection of the inner retina in macula which indicated hemorrhage. There
To investigate the efficacy of modified external dacrocystorhinostomy (DCR) with intubation in the treatment of lacrimal sac cyst. Twenty-three lacrimal sac cyst cases (23 eyes) were enrolled and treated with DCR technique and tube intubation. The success was assessed based on lacrimal irrigation and symptomatic relief of epiphora. Among 23 patients, surgery was interrupted and converted to dacryocystectomy in 1 case., thus surgery was successfully performed in 22 cases. Among them, 1 case lost follow up at 6 month, 1 case lost follow up at 12 month. At 1 month visit, lacrimal sac cyst disappeared in 22 cases. At 12 month visit, epiphora was completed relieved in 18 out of 20 cases, irrigation indicated no obstruction in all 20 cases. The modified DCR technique with intubation has satisfactory outcome for lacrimal sac cyst.
To evaluate the effect of intravitreal injection of triamcinolone acetonide(IVTA) combining with retinal laser treating for diabetic macular edema(DME). Twenty five patients(32 eyes) with DME who has microangioma in macula lutea were randomly divided into group A, B,C and D(8 eyes each group). Eyes in group A were treated with laser photocoagulation. Eyes in group B were treated with multiplier-532 laser photocoagulation and transpupillary thermotherapy. Eyes in group C were treated with multiplier-532 laser photocoagulation and intravitreal triamcinolone acetonide. Eyes in group D were treated with multiplier-532 laser, transpupillary thermotherapy plus triamcinolone acetonide injection. Intravitreal injection of 4 mg triamcinolone acetonide was done 1 week after laser photocoagulation in group C and D. The visual acuity, intraocular pressure, macular thickness (foveal thickness) of the eyes in 4 groups were observed before and 1, 3 and 6 months after treatment. The visual acuity, intraocular pressure and fo
To observe the changes of macular thickness in patients with sleep apnea syndrome (SAS) before and after sleep. Thirty-two patients (63 eyes) diagnosed as SAS from August 2003 to January 2007 were enrolled. Macular thickness were measured using optical coherence tomography (OCT) at 11:00 to 12:00 evening (before sleep)and 20 to 30 minute after sleep, respectively. The mean macular thickness was (123.00+/-19.98)um and (134.25+/-19.92)um before and after sleep, respectively. The mean difference was (11.25+/-9.04)um before and after sleep(t=9.878,p<0.05),95%CI(8.98,13.53)um. The macular thickness of SAS is increased in SAS patients, which may due to anoxia of SAS.
To explore the role of vascular endothelial growth factor and interleukin-6 in the aqueous humor in the pathogenesis of diabetic retinopathy(DR). Aqueous humor samples were collected in type 2 diabetic patients during cataract surgery. The classification of DR was assigned based on the fundus examination and fluorescein angiography after cataract surgery. The experiment group included non-diabetic retinopathy (NDR,21 cases),background diabetic retinopathy (BDR,26 cases) and Proliferative diabetic retinopathy (PDR,19 cases). The control group was the healthy cataract patients (20 cases). The levels of VEGF and IL-6 in the aqueous humor was assessed using the ELISA technique. The level of VEGF in NDR,BDR and PDR cases were(240.30+/-26.15)pg/ml, (292.27+/-58.91)pg/ml and (477.41+/-91.01)pg/m1, respectively. The level of IL-6 in NDR, BDR and PDR cases were (160.83+/-33.41)pg/ml, (238.60+/-62.23)pg/ml and (389.13+/-90.35)pg/ml,respectively. The levels of VEGF and IL-6 in the aqueous humour from the control group w
To study the expression of matrix metalloproteinase-2(MMP-2), matrix metalloproteinase-9(MMP-9) and vascular endothelial growth factor(VEGF) in retinoblastoma (RB) and its relationship with the differentiation and optic nerve infiltration of RB. Immunohistochemical technique was used to detect the protein expression of MMP-2, MMP-9 and VEGF in 40 RB cases. The MMP-2, MMP-9 protein and VEGF protein expression were detected as positive in 52.5%, 57.5% and 72.5% of 40 RB cases respectively. The expression of MMP-2, MMP-9 and VEGF was significantly higher in the undifferentiated pattern than in the rosetted pattern(p<0.05); and the expression of MMP-2, MMP-9 and VEGF was significantly higher in tumors with optic nerve invasion than in those without optic nerve invasion(p<0.05). In RB, the expression of MMP-2, MMP-9 had a positive correlation with the expression of VEGF. In RB, high expression of MMP-2, MMP-9 and VEGF is related to the invasion and metastasis of RB. The expression of MMP-2, MMP-9 is positively cor