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期刊名称: Trends in Biotechnology
Volume:15    Issue:6        Page:230-235
ISSN:0167-7799

Expanded-bed adsorption in industrial bioprocessing: Recent developments期刊论文

作者: Hjorth Rolf
DOI:10.1016/S0167-7799(97)01045-7

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页码: 230-235
被引频次: 170
出版者: Elsevier Ltd,ELSEVIER SCI LTD
期刊名称: Trends in Biotechnology
ISSN: 0167-7799
卷期: Volume:15    Issue:6
语言: English
摘要: Expanded-bed adsorption for the capture of proteins from feedstocks containing cells or cell debris has been used frequently during the last few years. The main reason for the increased number of examples is the availability of purpose-designed adsorbents and equipment for expanded-bed processes, making the technique efficient and compliant with the requirements of the pharmaceutical industry. Examples of processes where expanded-bed adsorption has been used to recover products from several-cubic-metre volumes of feedstocks now exist but there is still a need for the development of equipment and adsorbents that can handle even larger feedstock volumes. There is clearly a need to develop new adsorbents with increased protein-binding capacity. The use of highly specific and stable ligands in combination with improved adsorbents will make expanded-bed adsorption even more attractive. Such improvements will allow the initial recovery of products in industrial bioprocessing to be carried out in a shorter time with a high product yield, even when very large feedstock volumes are used.
相关主题: K DB BIOTECHNOLOGY & APPLIED MICROBIOLOGY, BIOTECHNOLOGY & APPLIED MICROBIOLOGY, ESCHERICHIA-COLI, PURIFICATION, FLUIDIZED-BED, PROTEIN RECOVERY, CELL-CULTURE, AFFINITY-CHROMATOGRAPHY, BROTH, Industrial Microbiology - trends, Animals, Fungal Proteins - isolation & purification, Bacterial Proteins - isolation & purification, Cell Culture Techniques - methods,

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